Animation showing how the Pippin Prep Runs to Automatically Select and Collect Different Sized DNA
25 Mar 2011
This video demonstrates how the Pippin Prep selects and collects 100bp, 200bp, 300bp and 400bp DNA fragments over 80 minutes. Gel Cassettes use an external DNA size standard run in a dedicated sample lane to determine the timing of size-selected collections. Four lanes are used for samples, and one extraction can be collected per lane according to user input size ranges. Sample lanes are physically separate and tapered to shorten run times and improve resolution.
About the company
Sage Science
Sage Science develops game-changing instrument products for sale to the Life Sciences research and Diagnostic laboratory markets. Our mission is to provide new systems to streamline and improve sample preparation workflows.
Sage Science is privately-funded. It is headquartered and manufactures at the Cummings Center in Beverly, MA, USA.
The Company released the Pippin Prep DNA size selection system in 2010. The Pippin has since established itself as the premium platform for high quality library construction for next gen sequencing applications.
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Sage ScienceThe Pippin Prep is a preparative electrophoresis platform for collection of size-selected DNA samples. The system automates DNA size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries. The system collects fragments (depending on gel type) between 90 bp- 8kb. A higher quality of sample can be extracted, more reproducibly and with no cross-contamination, when compared to manual gel purification methods.
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