Effects of the Velocity on the Pipette Shape
17 Feb 2016
This video illustrates the impact of changes in the velocity in the next-to-last line of a multi-line pull program. Starting point for those changes is sample program #1, which is covered in much detail in the tutorial video "Pulling a Patch Pipette from Quartz Glass". Learn how to recognize inappropriate settings of this parameter.
About the company
Sutter Instrument Co.
Sutter has developed a very strong reputation as a manufacturer of quality and innovative instrumentation at reasonable prices. Sutter's strong customer service orientation and technical expertise has led to the development of a very loyal customer base, exhibiting significant repeat purchasing patterns. In the area of micropipette fabrication, Sutter is the undisputed world leader in both instrumentation and technical knowledge. This has positioned Sutter at the forefront of emerging technologies that utilize micron and submicron pipettes and taper fiber optic probes. The synergy between Sutter's microprobe fabrication instruments, micropositioners and optical devices provides the company with a unique role in developing micromeasurement and nanofabrication technologies.
Related products
Request Quote for All Products
P-2000 Laser Based Micropipette Puller
Sutter Instrument Co.A significant advance in the technology of fabrication of micropipettes, optical fiber probes, and nanospray tips, is offered with the P-2000 micropipette puller. The P-2000 integrates a CO2 laser-based heat source with the technology derived from our extensive experience with conventional pullers. This system offers capabilities unmatched by other pullers. Key Features: Capable of pulling quartz, borosilicate and aluminosilicate glass. Fully programmable — including heating filament characteristics. The laser has no melting point limit as with conventional metal filaments; and therefore, cannot be burned out. Pulls electrodes with tip diameters smaller than 0.03µm. Optimized velocity sensing circuit for maximized sensitivity and reproducibility. Operating life of the CO2 laser is expected to be in excess of ten years with normal use, after which the laser can be refurbished by the Sutter Instrument Company for a nominal charge. Individual programs can be write-protected in order to secure them from inadvertent changes. The total time that the heat is on during the pull is displayed for improved program development and troubleshooting. A date and time stamp is displayed to show the last time that a program has been changed. The P-2000/F is ideal for applications such as nanospray and NSOM. Preprogrammed sample programs for intra-cellular and patch pipettes. Special programming available upon request.
