Top List: What's Hot in CRISPR Gene Editing Technology

From CRISPR screening to CRISPR/Cas9-edited fusion proteins, we discover the latest technology, news and products in gene editing technology

31 Jan 2019

Gene Editing and CRISPR Special Feature

The ability of the CRISPR-Cas9 gene editing system to enable high-specificity, site-specific double-stranded DNA breaks to effectively knockout a target gene, make precise base edits or stimulate gene activation or repression is continuing to revolutionize gene editing capabilities.  With the use of fluorophore tags, Cas9-fusion proteins and Cas9 alternatives, this technology can be incorporated into a range of applications, from drug discovery screening approaches to monitoring protein interactions and multiplex engineering.

In our Gene Editing and CRISPR SelectScience Special Feature this month, we explore the latest technology and guidance in the field. You can learn from the experts by watching our webinars on demand, downloading application notes, viewing the videos, and reading key articles for the field of gene editing. In this article, we pull together some of the very hottest topics in the field today. 

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Hot Topics in Gene Editing and CRISPR Technology:

Functional Genomic Screening for Whole Genome Investigation

CRISPR Screening Workflow

Functional genomic screening exploits genomic editing to induce a measurable phenotypic change in a variety of organisms and tissues. This is broadly applicable to drug discovery workflows, from novel target discovery to target validation and better identification of compound mechanisms of action. While there are a number of screening platforms available, it’s CRISPR technology that is opening up new applications of genomic screening.

In this interview, gene modulation and gene editing technology specialist Dr. James Goldmeyer describes the unique features offered by Horizon Discovery's functional genomic screening services.

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Genome-edited nanoBRET Expression SystemsCRISPR-Cas9 and nanoBRET technology

Bioluminescence resonance energy transfer (BRET) is a powerful tool for studying cellular protein interactions and trafficking. However, this technique is limited by the need for ectopic expression of labeled interaction partners. 

In this application note, we look at the activation and internalization of membrane-spanning G protein-coupled receptors (GPCRs) using CRISPR/Cas9-edited fusion proteins. Using CRISPR/Cas9 technology, DNA coding for nanoluciferase (Nluc) is successfully inserted into the genomic locus of CXCR4 to act as an endogenously-expressed, BRET donor-labeled fusion protein. Coupled with suitable acceptor fluorophores, this can be used in receptor internalization assays, detected by the CLARIOstar from BMG LABTECH.

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Groundbreaking Genetics

Learn how scientists at the UK's Institute for Genetic Medicine, in collaboration with the Wellcome Trust Centre for Mitochondrial Research, are using sophisticated consumable technologies to advance clinical research into rare mitochondrial diseases, paving the way towards major impacts on personalized medicine in mitochondrial and other rare diseases, as well as in cancer, Duchenne muscular dystrophy and an array of neurodegenerative disorders. 

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Dual CRISPRi and CRISPRa ScreeningDual CRISPR Screening

CRISPR-Cas9 technology can be incorporated into a range of screening approaches, from exploring drug mechanisms of action, to targeted drug resistance and identification of novel biomarkers. Depending on the expression effect required, there are three types of CRISPR screen available, which include:

  • CRISPR KO – target gene knockout
  • CRISPRi – target gene repression
  • CRISPRa – target gene amplification

In this application note by Horizon Discovery, we look at the combination of all three approaches (CRISPR KO, CRISPRi and CRISPRa) to identify components that influence resistance and sensitivity to the standard of care BRAF inhibitor, vemurafenib.

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Explore our selection of the biggest news stories in NGS:

  1. Horizon Discovery extends CRISPR Screening Service to Primary Human T cells
  2. Locate Bio Granted Key US & EU Patents for its IntraStem Non-Viral Next-Generation Gene Delivery Technology
  3. Gene Therapy is Closer Than We Think – A Success Story
  4. Microenvironment and 3D Image Analysis Key in the Development of More Efficient Drugs Against Complex Diseases
  5. DefiniGEN Licenses CRISPR-Cas9 Gene Editing Technology to Develop Cell Models for Optimized Metabolic Disease Drug Development
  6. CRISPR Therapeutics and ProBioGen Collaborate to Develop Novel In Vivo Delivery Technologies
  7. Is It Possible to Predict The Future of Science?

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