Search results for "flow cytometry"

Lentiviruses are commonly used as viral vectors in cell and gene therapy production, delivering and steadily integrating desired genes into the genome of the host cell. However, they may also present health hazards to humans due to integration-mediated transformation and the creation of a RCL that can infect nontarget cells. While the current regulatory guidelines recommend a cell culture–based method for RCL testing, it is cu…

Adeno-associated virus (AAV) is commonly used as a viral vector in cell and gene therapy production, but there is a risk of it becoming a replication competent virus (RCV) during the production process, especially in the presence of a helper virus, which can raise safety concerns. While the guidelines recommend a cell culture–based method for RCAAV testing, it is cumbersome and takes 30–45 days from sample to results. Verichec…

Heating elements embedded in grooves radiating freely into the furnace chamber give these models particularly short heating times. Thanks to their robust lightweight refractory brick insulation, they can reach a maximum working temperature of 1300 °C. These models thus represent an interesting alternative to the familiar L(T) 3/11 models, when you need particularly short heating times or a higher application temperature. T…

These UV Sterilisation Cabinets provide a convenient area for setting up PCR reactions in a nucleic acid free environment thus limiting PCR reaction contamination. Acting effectively as a low cost alternative to a clean room, the powerful UV lights on the cabinets denature nucleic acids in 5 to 30 minutes making them unsuitable for amplification. The cabinet incorporates safety features to prevent user exposure to UV light. Th…

The kit is designed for the efficient and convenient isolation of PCR products from amplification reactions. Primers, mineral oil, salts, unincorporated nucleotides, and the thermostable DNA polymerase may inhibit subsequent enzymatic reactions (e.g., labeling, sequencing or cloning of the PCR products). In addition, nucleic acids from other modification reactions (e.g., restriction-endonuclease digests, alkaline-phosphatase t…

Proteema is the right choice for the separation of all kinds of biomolecules including proteins and gelatines. The columns are silica based and can be used with eluents up to a pH value of 7. If the application requires separations at higher pH in more ‘basic’ eluents, PSS SUPREMA a polymer based material is recommended. Generally the pore-size distribution of silica based columns is smaller than that of polymeric columns, whi…

Feel the need for speed in your lead development time? The Answer – High Through Put Screening ATPase or GTPase Assays from Innova Biosciences. Is your drug discovery program for ATPase or GTPase enzymes holding you back? Still searching for an inhibitor? Then you need Innova Biosciences easy to use, GTPase or ATPase assay kits. The non radioactive colorimetric assay kits use a 96 well format, and all the necessary reagents ar…

Reveleris® Amino and Diol Flash Cartridges Silica flash cartridges, while effective for many applications, have their limitations for some compound classes like carbohydrates and lipids. For these applications, Grace Davison Discovery Sciences has introduced the Reveleris® Amino and Diol Flash Cartridges. Reveleris® Amino Flash Cartridges • Ideal for polar and basic molecules • Compounds such as carbohydrates, nitrogen contain…

Bioneer’s AccuPrep® Viral RNA Extraction Kit is the ideal solution for rapid extraction of viral RNA from cell-free samples such as serum, plasma, CSF, urine, and from a variety of RNA virus, such as HIV, HAV, HCV and enteroviruses. The viral RNA extracted with the AccuPrep Viral RNA Extraction Kit can be directly used as a template for RT or RT-PCR. AccuPrep Viral RNA Extraction Kit obtained has an average A260/A280 ratio gre…

Bubbles in perfusion systems are caused by solution temperature changes. As solutions warm, they cannot hold as much gas, so they off-gas. This forms bubbles in perfusion tubing. It is especially true in inline heaters where the temperature rises dramatically. The best cure for bubbles is to pre-warm your solutions. AutoMate Scientific introduces the BubbleStop™ Syringe Heater with integrated temperature sensor and feedback. S…

Bubbles in perfusion systems are caused by solution temperature changes. As solutions warm, they cannot hold as much gas, so they off-gas. This forms bubbles in perfusion tubing. It is especially true in inline heaters where the temperature rises dramatically. The best cure for bubbles is to pre-warm your solutions. AutoMate Scientific introduces the BubbleStop™ Syringe Heater with integrated temperature sensor and feedback. S…

Convenient and reliable concentration, purification, and diafiltration of 20 to 60 mL biological samples with Pall Life Science's Jumbosep Advance Centrifugal Devices Features of the Macrosep Advance Centrifugal Devices:  •  Typically concentrates 60 mL sample volumes to 5 mL in 30 minutes.    •  Provides high recoveries, typically > 90%.   •  Low protein-binding Omega membrane and polypropylene housing minimize losses due to…

The new Finnigan Pocket Surveyor software providing local instrument control for new and existing Finnigan Surveyor HPLC Systems. The software operates on a handheld computer running the popular Windows Pocket PC 2002 O/S and offers chromatographers the ability to connect directly to their Surveyor quaternary gradient pump and/or variable wavelength detector.   In addition to individual module control, the Finnigan Pocket Sur…

The ideal companions for QC/QA laboratoriesQsurf is an ultra-fast surface area and total pore volume analyzers family based on the flow dynamic adsorption technique. It is available in four different configurations, single and multi point BET, with either one or three analytical ports. Despite the speed of analysis and the high throughput, Qsurf analyzers provide accurate determinations thanks to the sensitivity of its TCD det…

The LyoStar3 is designed to meet the needs of R&D professionals. Our freeze drying equipment is used for applications ranging from process development for scale-up to product processing for stability studies to formulations work. The LyoStar is an R&D and process development freeze-dryer that provides unmatched process accuracy, reliability and unique ease-of-use features. The LyoStar features Type T thermocouple-controlled s…

The Movable Objective Microscope (MOM) is designed to be an ultra-stable, versatile, fluorescence platform for electrophysiology experiments. The MOM is a new alternative to the popular technique employing a fixed-stage, upright microscope on a translating X-Y table. The advantage of the MOM is that only the microscope objective is moving in 3 dimensions. The main frame of the microscope, including the light source and camera…

The TargetLynx™ Application Manager automates sample data acquisition, processing and reporting for quantitative results.It incorporates a range of confirmatory checks that identify samples that fall outside user-specified or regulatory thresholds. Identify Non-compliant Samples TargetLynx rapidly identifies and flags samples where: Analytes are above a specified maximum reporting level (MRL) Analyte confirmatory ion rat…

The SVG-SYS Vari-gel MINI System is ideal for standard preparatory and analytical electrophoresis techniques.Colour-coded combs of 1, 1.5 or 2mm thickness provide a maximum 48-sample throughput. Two UV-transparent gel-casting trays of dimensions 7 x 7 and 7 x 10cm allow nucleic acids to be resolved over longer and shorter distances as preferred, as well enabling the electrophoresis tank to remain in use during casting.SVG-SYS…

The TVG-SYS Vari-gel MIDI System is ideal for standard preparatory and analytical electrophoresis techniques. Colour-coded combs of 1, 1.5 or 2mm thickness provide a maximum 80-sample throughput. Three UV-transparent gel-casting trays of dimensions 10 x 7, 10 x 10 and 10 x 15cm allow nucleic acids to be resolved over longer and shorter distances as preferred, as well enabling the electrophoresis tank to remain in use during c…

The VG-SYS 15cm wide Vari-gel MAXI System is ideal for standard preparatory and analytical electrophoresis techniques. Colour-coded combs of 1, 1.5 or 2mm thickness provide a maximum 180-sample throughput. Four UV-transparent gel-casting trays of dimensions 15 x 7, 15 x 10, 15 x 15 and 15 x 20cm allow nucleic acids to be resolved over longer and shorter differences as preferred, but also enable the electrophoresis tank to rem…

A 48 um soft non-permeable aluminum foil sealing film with 30 um layer of strong acrylic adhesive. FoilSealTM sealing foils remove cleanly from plate surfaces with no adhesive stringing, residue or contamination. Each sealing foil measures 78.3 x 129 mm and offers sufficient sealing area for all plate types (e.g. flat top and raised-rim). Chamfered corners result in no plate overhang for robotics applications. Features one end…

Symyx Software Analytical Notebook is an Electronic Laboratory Notebook (ELN) specifically designed to enable analytical chemists to use prior experience, develop new methods, access relevant information, and integrate to critical systems to be more efficient and effective. Prepare: Design experiments and data templates Flexible data entry tools allow scientists to plan all types of experiments, including method developme…

R & D Systems Active STAT3 DuoSet IC® Kit - For the development of transcription factor assays to measure human and mouse active STAT1 p91. The R & D DuoSet IC Phospho-specific ELISA format is a sensitive and convenient phosphorylation assay for use with cell lysates. The DuoSet IC signal transduction assays are an excellent alternative to Western blot, especially when used in combination with DuoSet IC ELISAs designed to mea…

The MT-1000 is a microscope translator, fixed-stage and manipulator platform for moving optical pathway electrophysiology. The modular design of the MT-1000 features freestanding columns that provide rock solid mounting for the tissue recording chamber and multiple micromanipulators, such as the MP-285 or MP-225. The columns are positioned around a movable table that provides X-Y translation of the optical pathway in order to…

The CHEMICON® QCM™ Chemotaxis 5 μm 24-well Migration Assay is performed in a Migration Chamber, based on the Boyden chamber principle. The 5 μm pore size of this assay's Boyden chambers is appropriate for studying monocyte/macrophage migration. The quantitative nature of this assay is useful for screening of pharmacological agents. Each kit provides sufficient materials for the evaluation of 24 samples. The Chemicon QCM™ Chemo…