The mag™ kits use magnetic beads to purify DNA from a variety of sample types. They are a cost-effective solution for automated DNA extractions using magnetic particle handlers, as well as liquid handlers. The protocol follows the common technology of binding the DNA to the mag beads, removing contaminations by salt and alcohol based wash buffers and a final eluation step.
Control set containing rat and mouse insulin and rat C-peptide to be used in the rat and mouse insulin kits as well as in Rat C-peptide ELISA. Perform the Mercodia ELISA according to the "Directions for Use", handling Diabetes Antigen Control Rat&Mouse (Low, Medium, High) as samples. The controls contain rat insulin, mouse insulin, and rat C-peptide
The Agilent RNA kits with the patented RNA Integrity Number (RIN) are the industry standard for RNA quality assessment. Do fast, easy and precise integrity checks and sample quantitation before any RNA dependent application. Maximum flexibility €“ check the quality of your total RNA, mRNA, or cRNA samples Minimal sample consumption €“ use as little as 5 ng of total RNA or 50 pg of total RNA for analysis, savi…
Agilent Gene Expression Hybridization Kits offer consistently more reliable and reproducible microarray results through robust fluorescently-labeled cRNA hybridization. The kit has been optimized with Agilent gene expression microarrays, balancing hybridization temperature, oligo design and components of hyb buffer to maximize specificity and sensitivity of the assay. We provide different kit sizes to respond to different t…
KAPA SYBR FAST One-Step qRT-PCR Kits are designed for optimal performance in stringent qRT-PCR reaction conditions, exhibiting dramatic improvements in sensitivity, specificity, reaction efficiency, and fluorescence. The kit is optimized for rapid one-step, one-tube RNA quantification, reducing experimental variation and contamination with a convenient qRT-PCR protocol. KAPA SYBR FAST One-Step qRT-PCR Kits contain M-MuLV r…
The Mosaic ELISA Human Growth Factor Panel 1 is an excellent tool for the detection of 6 different growth factors in the same sample. Mosaic ELISA kits employ multiplex microarray technology to provide an accurate, efficient, and economical alternative to conducting multiple traditional ELISA experiments. Growth factor is a term used to describe a broad range of structurally diverse molecular families and individual proteins b…
Guava Technologies' ViaCount assay is revolutionizing the way cell counting and viability assessment are done in today's laboratories. Fast, automated, and highly reproducible, the Guava ViaCount assay addresses many of the difficulties of conventional cell counting and viability assessment methods. The Guava ViaCount assay provides: Cell counts up to ten times faster than manual counting, in a convenient turnkey assay.…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
The Rac1/Cdc42 Activation Assay provides an effective method for detecting Rac and Cdc42 activity in cell lysates. This assay uses the downstream effector of Rac/Cdc42, p21-activated protein kinase (PAK1), to isolated the active GTP-bound form of Rac/Cdc42 from the sample. The p21 binding domain (PBD) of PAK1 is expressed as a GST-fusion protein and coupled to agarose beads. After precipitation, an immunoblot is performed and…
Chemicon's membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of FPR1 and its ligands. The membrane preparations exhibit a Kd of 0.39 nM for [125I]-WKYMVm. With 5 μg/well FPR1 Membrane Prep and 0.5 nM [125I]-WKYMVm, a greater than 20-fold signal-to-background is…
Chemicon's V1A membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of V1A interactions with its ligands. The membrane preparations exhibit a Kd of 55 pM for [125I]-vasopressin antagonist ([125I]-phenylacetyl-D-Tyr(Me)-Phe-Gln-Asn-Arg -Pro-Arg-Tyr-NH2). With 10 µg/well V1A Membr…
The MegaMan human transcriptome library is a collection of cDNA created using mRNA from 66 diverse sources, including 32 from human tissues and 34 from human cancer cell lines. This human transcriptome library is ideal for cloning both well-characterized genes and transcripts previously identified by expressed sequence tag (EST) studies. Largest comprehensive collection of the human transcriptome in a single tube Enriched…
The MegaMan human transcriptome library is a collection of cDNA created using mRNA from 66 diverse sources, including 32 from human tissues and 34 from human cancer cell lines. This human transcriptome library is ideal for cloning both well-characterized genes and transcripts previously identified by expressed sequence tag (EST) studies. Largest comprehensive collection of the human transcriptome in a single tube Enriched…
Bio S&T is the only commercial provider of highly purified human tRNAs. RNA is most widely used as the primer of retroviral reverse transcriptases. The 3’ end of the tRNA binds to the complementary binding site on the viral RNA therefore initiating reverse transcription. 1. tRNALys3 is the primer of HIV-I, HIV-II, SIV and FIV. 2. tRNALys1,2 are the primers of BLV, HTLV-I and II, SSV, FLV, VV, CAEV, MPMV, SMR and EIAV. 3. tRNAT…
May be used in a variety of laboratory assays Flat bottom strips are designed to easily break apart and may be used in an style strip holder that allows each individual well to be positioned back into the plate once broken High binding surface binds medium (>10kD) and large biomolecules that possess ionic groups and/or hydrophobic regions. Binding capacity is approximately 400 to 500ng IgG/cm2. Ideal for immunoassays Reco…
Activation of p38 MAP kinase is measured by an increase in fluorescence in the nucleus of celsl. This kit includes antibodies, buffers, and a complete protocol for preparation of imaging-quality cells in microplates. The secondary antibody is conjugated to Alexa® Fluor 488 for optimal detection of phospho-p38 with a FITC emission filter. The kit has been validated for use on Cellomics’ ArrayScan® HCS Reader but may be appli…
(This is the suggested replacement for 3851)Excellent signal dynamic range due to low background fluorescence and enhanced signal intensitySuperior flatness -minimizes variability within a plate and between platesFlat bottom wells are compatible with automated dispensers including the PerkinElmer FlexDrop™Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interac…
(This is the suggested replacement for 3852)Excellent signal dynamic range due to low background fluorescence and enhanced signal intensitySuperior flatness -minimizes variability within a plate and between platesFlat bottom wells are compatible with automated dispensers including the PerkinElmer FlexDrop™Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interac…
Round well design with a solid black flat bottom 2.8mm distance between well bottoms to plate bottom Validated by Molecular Devices for the FLIPR reader. Outer wells aligned for proper viewing with FLIPR Ideal for miniaturized 384 well fluorescent homogeneous assays Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interactions Suitable primarily for the…
No logo or lettering for improved heat sealing and ease of liquid handling Low base designed for optional bottom reader sensitivity Excellent signal dynamic range due to low background fluorescence and enhanced signal intensity Superior flatness -minimizes variability within a plate and between plates Flat bottom wells are compatible with automated dispensers including the PerkinElmer FlexDrop™ Not treated (or medium bi…
Cap Translucent flasks are supplied with a PFA screw cap. Screw caps provide hermetic seals and help reduce the possibility of outside contamination. The product is manufactured to Class A tolerances as defined by ISO 1042 and flasks have individually calibrated graduation lines. They withstand high temperatures and are resistant to a variety of aqueous solutions and organic solvents. These flasks may be autoclaved to 121ºC wi…
IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It is an engineered recombinant protease overexpressed in E. coli that cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments. The protocol for a standard reaction is to add the IdeS Protease to the IgG sample, add 1 unit of IdeS Protease per 1µg of IgG to be digested…
(This is the suggested replacement for 3711) Ideally suited for fluorescent assays using either top or bottom detection plate readers Recommended working volume of up to 20 to 80 µL Conforms to standard microplate footprint and dimensions Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interactions Is suitable primarily for the immobilization of large mol…
(This is the suggested replacement for 3706)25 per Bag, without Lid, NonsterileIdeally suited for luminescent assays using either top or bottom detection plate readers Recommended working volume of up to 20 to 80 µL Conforms to standard microplate footprint and dimensions Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interactions Is suitable primarily for th…
Not treated (or medium binding) polystyrene surface is hydrophobic in nature and binds biomolecules through passive interactions Is suitable primarily for the immobilization of large molecules, such as antibodies, that have large hydrophobic regions that can interact with the surface Binding capacity of approximately 250 ng of mouse IgG/cm2 Certification results for each lot are made available upon request by contacting l…
