White solid. Aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3¡ä-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Intro…
Light brown lyophilized solid. Working concentration 50 mg/ml - 1 mg/ml for mammalian cell selection. Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isol…
The ProteoPlex™ 16-Well Murine Cytokine Array Kit is designed for multiplex detection and measurement of 10 important mouse cytokines in parallel from up to 15 experimental samples. The 10 analytes measured in the kit are pro- and anti- inflammatory cytokines important for the study of immune system regulation and diseases such as asthma and arthritis. Each well on the slide contains a microarray of spotted antibodies with fou…
The ProteoPlex™ 16-Well Human Cytokine Array Kit is designed for multiplex detection and measurement of 12 important human cytokines in parallel from up to 15 experimental samples. The combination of advanced surface chemistry, well-characterized capture agents, and a highly-sensitive fluorescence-based detection system provides a robust method for comparing relative cytokine abundance or cytokine quantification in serum sampl…
Novel protein markers for visible tracking & accurate sizing on any Western blotTrail Mix™ Western Markers consist of the Perfect Protein™ Western Markers supplemented with a group of three prestained indicator proteins to allow direct visualization of protein mobility during electrophoresis. Trail Mix Western Markers and Western Blot Kits have all of the features of Perfect Protein Western Markers and Western Blot Kits, plus…
The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of Novagen's expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10 to 8–1 × 10–9 M) enables sensitive, specific detection of His•Tag fusion prote…
Benzonase Nuclease - Effective viscosity reduction and removal of nucleic acids from protein solutions Benzonase Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. Benzonase Nuclease is effective over a wide range of conditions and possesses an exceptionally high specif…
Purification of His•Tag fusion proteins by metal chelation chromatographyThe His•Bind® family of products offers a wide selection of supports designed for rapid one-step purification of proteins containing the His•Tag® sequence by immobilized metal affinity chromatography (IMAC). The His•Tag sequence (6, 8 or 10 consecutive histidine residues) binds to divalent cations (Ni2+) immobilized on NTA*- and IDA-based His•Bind resins.…
The most common method for transformation of plasmids into E. coli is to use chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility and convenience are achieved using prepared competent cells. Novagen offers the widest selection of competent cells available for protein expression. Our strains offer a variety of options that enable you to maximize the yield an…
Top quality inhibitors for all aspects of biological research. The Calbiochem® range includes inhibitors of Phosphorylation/dephosphorylation ApoptosisCell Division/Cell Cycle/Cell Adhesion Lipid Signalling Neurobiology/Neurodegeneration Nitric Oxide/Oxidative Stress Proteases
More than 3000 monoclonal and polyclonal, primary and secondary antibodies. The Calbiochem® range (incorporating Oncogene Research Products™) now includes the most comprehensive selection of antibodies to MMP’s, G-proteins and Phospho-Proteins on the market today. The extensive range also covers protein kinases, apoptosis, calcium signalling, cancer/cell cycle research, angiogenesis, growth factors and much more. Many product…
Pseudoproline dipeptides are undoubtedly the most powerful tools described to date for enhancing synthetic efficiency in Fmoc SPPS. Novabiochem® now offers 28 pseudoproline dipeptides - representing the widest selection on the market today The routine use of pseudoproline dipeptides has been found to: Avoid costly and unnecessary repeat syntheses of failed sequences Increase purity of crude products Simplify HPLC puri…
Two new additions to the SNAP CAP™ range. These caps are pre-cut to avoid any vacuum generation problems.
Calbiochem® supplies a wide variety of products for the study of apoptosis, includingActivators and Inhibitors Antibodies and Blocking Peptides Assay Kits Caspase-related products Mitochondrial Permeability-Transition Reagents and Related Products Poly(ADP-Ribose) Glycohydrolase (PARG) Inhibitors Poly(ADP-Ribose) Polymerase (PARP) Inhibitors Telomerase Products View the online interactive pathways to easily locate the product…
The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Based on the T7 promoter-driven system, Novagen’s pET System has been used to express thousands of different proteins. pET Vectors are supplied as purified plasmid DNA (10 µg) plus an Induction Control strain (supplied as a glycerol stock). Complete sequences and detailed vector maps are available.
Complete range of Calbiochem® high quality detergents including Bile Salts Detergent Adsorbents Detergent Kits Ionic Non-Detergent Sulfobetaines (NDSB) Non-Ionic Zwitterionic
The D-Tube™ Dialyzers can be used for dialysis and electroelution of proteins or nucleic acids from polyacrylamide or agarose gels. The disposable, single-use tubes require no syringes, microcentrifuges, or laborious steps to manipulate small samples volumes. The sample is added and removed using a standard laboratory pipette. Available with molecular weight cut-offs from 3.5 to 14 kDa, the D-Tube Dialyzers are designed in thr…
Understand and manage the performance of your automated liquid handlers, multichannel pipettes, labware, operators, and more.
Small Chromatography Samples? Big celebration! Champagne vials from LabHut are the ideal economical solution when small volumes are to be injected. Costing less than the price of an insert alone, Champagne vials can contain as much as 1.5ml of sample and yet have a residual volume of just a few microlitres. Available in clear or amber glass, with a choice of crimp, snap or screw caps, the vials are compatible with virtual…
This waterproof, battery-operated conductivity meter stands up to the most demanding soil and water analysis applications and delivers over 2,000 hours of continuous operation. Convenient Quick-Start Guide is located on the back of the meter to make the unit easy to operate in the field. The rugged meter displays conductivity, resistivity, salinity, TDS and temperature measurements and offers a full complement of GLP-relat…
This competitively-priced, single-channel dissolved oxygen meter is easy to set up and use while providing reliable, accurate DO measurement for water analysis and general laboratory applications. The rugged meter displays DO concentration or percent DO Saturation and offers a full complement of GLP-related features. It offers enhanced stability indicators, salinity correction and automatic barometric pressure compensation—…
This competitively-priced, single-channel conductivity meter is easy to set up and use while providing reliable, accurate conductivity, resistivity, salinity, TDS and temperature measurements for soil and water analysis applications. The rugged meter accepts both 4-electrode and 2-electrode cells and offers a full complement of GLP-related features. SMART STABILITY™ and SMART AVERAGING™ functions simplify the measurement pro…
This waterproof, battery-operated DO meter stands up to the most demanding water analysis applications and delivers over 2,000 hours of continuous operation. Convenient Quick-Start Guide is located on the back of the meter to make the unit easy to operate in the field. The rugged meter displays DO concentration or percent DO Saturation and offers a full complement of GLP-related features. It offers enhanced stability indica…
