Wet or Dry? Which Type of Transfer is Best for Your Protein?

To perform a Western blot, proteins are separated according to molecular weight by electrophoresis through a polyacrylamide gel. The proteins are then transferred from the gel onto a solid membrane support for subsequent immunodetection by protein-specific antibodies. Transfers can be performed by wet transfer or under semi-dry conditions. This application note outlines the benefits and drawbacks of each system which must be considered when optimizing transfer conditions.