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Vitamin D and Metabolites: Evaluation of Supported Liquid Extraction (SLE) Prior to LC-MS/MS Analysis

24 Oct 2011

This application note demonstrates the extraction of vitamin D using 96-well supported liquid extraction (SLE) prior to LC-MS/MS analysis. It leads the reader through the various stages of analysis from sample preparation with SLE through to the ideal conditions required for HPLC and MS. It finally presents the results found and conclusions drawn from the study.

ISOLUTE SLE+ Supported Liquid Extraction Products

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ISOLUTE® SLE+ plates and columns enable efficient supported liquid extraction of analytes from whole blood, plasma, serum, urine, and oral fluid. The simple load-wait-elute method delivers 99.9% removal of proteins and phospholipids without offline steps, offering superior recovery, reproducibility, and cleaner extracts than liquid-liquid extraction.

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Vitamin D and Metabolites: Evaluation of Supported Liquid Extraction (SLE) Prior to LC-MS/MS Analysis