Utilizing the multiparameter capability of the ZE5 cell analyzer to monitor T cell exhaustion and effects of immunotherapy

T cell exhaustion is a hyporesponsive state of T cells characterized by sustained expression of suppressive cell surface markers, decreased effector cytokines, and impaired cytotoxicity. In this application note, Bio-Rad monitor in-depth changes to the T cell phenotype, exhaustion, and cellular subset identity markers using a 16-parameter panel developed for use with a 5-laser ZE5 Cell Analyzer.