Using a next-generation high-content screening platform and AI-analysis tools to increase insights from the complex 3D assay

This application note demonstrates how Molecular Devices’ ImageXpress^® HCS.ai system, combined with AI-powered IN Carta^® analysis, delivers high-quality 3D imaging and phenotypic insights. Using patient-derived colorectal cancer organoids, the workflow enables precise segmentation, robust classification, and quantification of drug effects in complex biological assays.