Use- and State-Dependent NaV 1.5 Blockers on QPatch X and In Vivo and In Vitro Assays
27 Mar 2011This application poster shows that the QPatch X in multi-hole mode can be successfully used for screening NaV1.5 blockers. The cardiac voltage dependent sodium channel (NaV1.5) is responsible for the upstroke and directed propagation of action potentials in the heart, and is therefore a central ion channel in safety assessment and drug discovery. For drug screening, a protocol is established for QPatch X that both tests the decay of the sodium current (30 Hz pulsetrain), and the recovery of the current from this pulse-train induced decay.
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QPatch HT and QPatch HTX
Sophion BioscienceThe QPatch HT and QPatch HTX enable high-throughput patch clamping with 48 parallel recording sites. Automated patch clamping with the same high quality afforded by conventional patch clamping can now be applied in all phases of ion channel drug discovery, including primary screening of focused compound libraries. • Up to 7000 data points per day • Screening and compound profiling • Multi-hole and single-hole mode (QPatch HTX) • Ligand- and voltage-gated recordings • Eight pipette liquid handling with intelligent scheduler • True gigaseals • Flow channels for solution exchange • Up to 50 liquid additions • User-friendly experiment execution • Unattended operation for several hours • On-board cell preparation • Elevator units for QPlate stacking • Elevator units for compound plate stacking • Cell clone screening feature • Multiple intracellular solutions testing feature • User friendly assay setup and quick data analysis