ResourceLife Sciences
Unlock the power of In-Cell Westerns with total cell staining and advanced imaging
4 Jul 2024In-Cell Westerns (ICW) have emerged as a powerful and versatile technique, facilitating the study of cellular behaviors at a molecular level. The ICW assay is a fluorescence-based immunodetection technique that combines the principles of immunofluorescence and Western blotting, allowing researchers to probe specific target proteins within whole cells. Azure Biosystems conducted an experiment looking into the potential of this technique in the study of intricate cellular responses. Discover how combined with the Sapphire™ FL Biomolecular imaging system with high sensitivity and minimal cross-talk, ICW becomes a dynamic and versatile tool, capturing subtle variations in protein expression and treatment response.
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ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ReagentsCell StainingFluorescence Analysis
