TSA Reduces Exposure Time in an Imaging Assay for PKCa

Protein kinase C alpha (PKCa) is associated with a wide variety of cellular processes including proliferation, adhesion and motility. Over expression of PKCa appears to be associated with certain forms of cancer, and it has been identified as a biomarker for poor prognosis in breast cancer. Upon activation with phorbol esters, PKCa translocates from the cytosol to the plasma membrane. For this reason, it is best studied with a high content analysis method that provides quantitative data on subcellular localization. In this application note read about Tyramide Signal Amplification™ (TSA), a widely referenced technology for providing greatly enhanced sensitivity in an extensive range of assays including immunohistochemistry and immunofluorescence (IF).