Trace-Level Analysis of Metanephrines in Plasma by HILIC LC-MS/MS
13 Dec 2018Highly sensitive analysis of metanephrines in plasma is critical in the diagnosis and treatment of pheochromocytoma and paraganglioma. Here, a HILIC LC-MS/MS method was developed using a Raptor HILIC-Si column because this approach provides retention of all target compounds in highly organic mobile phases, thus providing the increased sensitivity needed to reach low detection limits. Data from linearity, accuracy, and precision testing demonstrate that accurate results are consistently obtained—even at trace levels—for these important clinical biomarkers.
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Raptor HILIC-Si LC Columns
Restek Corp.Switch to a Raptor HILIC-Si LC column when you need more retention and resolution of hydrophilic, polar analytes. HILIC silica column designed for increased retention of polar compounds without ion-pairing reagents. 2.7 μm Raptor core-shell particles provide the speed of SPP, with greater efficiency and capacity than 5 μm particles. Ideal for increasing sensitivity and selectivity in LC-MS analyses. Fully compatible with both HPLC and UHPLC when using low backpressure HILIC mobile phases.