Thermo Scientific Solaris RNA Spike Control Kit: Identifying Reaction Inhibition in the RT-qPCR Workflow

20 Dec 2013

This application note describes the utility of the Solaris RNA Spike Control Kit to identify reaction inhibition and provide a complementary technology to better understand and interpret RNA quality. Experimental results demonstrate the Spike Control Kit accurately identifies RT-qPCR inhibition in the presence of heparin, lysis buffer and phenol.

Solaris qPCR Expression Assays

Thermo Fisher Scientific

Pre-designed, gene-specific probe and primer pairs for repeatable, sensitive and gene specific RT-qPCR quantification for human and mouse protein-coding genes and long noncoding RNAs (lncRNAs). Thermo Scientific Solaris qPCR Gene Expression Assays are pre-designed, gene specific probe and primer pairs that utilize minor groove binder (MGB) and Superbase technologies to deliver repeatable, sensitive and gene specific RT-qPCR (real-time) quantification for human and mouse protein-coding genes and long noncoding RNAs (lncRNAs).Solaris Assays are pre-designed to specifically detect all known variants of a gene with a single assay for high-confidence results. Solaris qPCR Primer and Probe Sets should be used in conjunction with the complementary instrument-specific Solaris qPCR Gene Expression Master Mix for optimal performance.Solaris qPCR Assays have also been pre-designed to the most commonly used reference genes to help you normalize expression levels of your experimental samples.Solaris qPCR Expression Assays Features & Benefits: High performance assay – Expect excellent results in terms of repeatability, sensitivity, dynamic range, efficiency and specificity. Sequence information included – Probe and primer sequence information is provided with every assay so you can publish with confidence. The master mix is blue – Solaris assays have been developed with our optimized, blue qPCR master mix for ultimate assay performance and ease-of-use. See your reagents as you pipette, track your progress across multiple wells, and visually assess your set-up. Familiar protocol – If you currently use qPCR probe assays, Solaris Assays will not require major changes to your protocol or routine.

(0)

Links

Tags

Thermo Scientific Solaris RNA Spike Control Kit: Identifying Reaction Inhibition in the RT-qPCR Workflow