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The Determination of Total Protein Using the LAMBDA UV/Vis Spectrophotometer: Bradford Method
29 Mar 2016This application note describes use of the Bradford protein assay, a spectroscopic analytical procedure used to determine the concentration of protein in a solution. In this study the LAMBDA™ 465 UV/Vis Spectrophotometer from PerkinElmer was used with the UV Lab™ Software. The results from the study show this method obtains good sensitivity and has rapid acquirement of spectra.
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LAMBDA 25, 35, & 45 UV/Vis Spectrophotometers
PerkinElmerPerkinElmer's LAMBDA 25/35/45 UV/Vis systems are easy to operate and deliver trustworthy results with the minimum of operator training. With over 12,000 units installed worldwide, our superior technology and proven build quality has proven itself under the toughest conditions. Our extensive suite of Instrument Performance Verification (IPV) tests confirms high quality performance over the lifetime of the system, and for regulated industries the Enhanced Security (ES) version of UV WinLab integrates seamless 21CFR part 11 technical compliance.LAMBDA 25Choose the LAMBDA 25 for routine UV/Vis applications including liquids analysis, pharmacopeia and regulatory tests. Range: 190 nm - 1100 nm Bandwidth: 1 nm (fixed) LAMBDA 35Choose the LAMBDA 35 for measurements on liquids, solids, pastes and powder samples and regulatory tests requiring variable bandwidths. Range: 190 nm - 1100 nm Bandwidth: 0.5 nm - 4 nm (variable) Integrating sphere option LAMBDA 45Choose the LAMBDA 45 for measurements on turbid and light-scattering liquid samples such as biological solutions and suspensions. Range: 190 nm - 1100 nm Bandwidth: 0.5 nm - 4 nm (variable) Pre-monochromator for reduced stray light
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