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The advantages of direct digital dispensing over manual serial dilution

10 May 2020

In this application note, Tecan discusses the advantages of Direct Digital Dispensing compared to manual serial dilution by comparing the results of dose response curves set up using the Tecan D300e Digital Dispenser and a traditional manual serial dilution method in two different assays: the LANCE® cell-based cAMP assay and a biochemical assay for nuclear receptor activation by AlphaScreen®.

D300e Digital Dispenser

Tecan

The D300e Digital Dispenser* uses direct digital dispensing technology to enable rapid delivery of picoliter to microliter sample volumes. It delivers fast, reliable performance across a large dynamic range, eliminating the need for slow, error-prone and wasteful serial dilutions for creation of dose-response curves and enabling miniaturized qPCR reaction set-up.

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The advantages of direct digital dispensing over manual serial dilution