SelectScience
Join Free
ResourceLife Sciences

Technical Parameters for the Use of Corning® DNA-BIND™ Products in High-Throughput Screening

Corning Life Sciences
23 Dec 2014

Previous work has demonstrated the use of DNA-BIND products for hybridization to immobilized capture probes, solid-phase mini-sequencing, examining DNA-protein interactions, and kinase assays. This application note demonstrates that Corning DNA-BIND products can facilitate the development of high-throughput solid-phase assays to analyze interactions between numerous biomolecules and provides technical parameters for their use.

Related products

Request Quote for All Products

DNA-BIND® 1 x 8 Stripwell™; 96-well Polystyrene Microplate without Lid, Nonsterile

Corning Life Sciences

Clear flat bottom polystyrene strips are designed to easily break apart and are pre-assembled in an “egg-crate” style strip holder that allows each individual well to be positioned back into the plate once broken. DNA-BIND® surface covalently binds to amine groups, and provides a convenient method to covalently immobilize aminated ssDNA by either the 5’ or 3’ end for hybridization and/or amplification, and for other DNA-based assays. Stripwell plates have: -Flat well bottoms. -Total well volume of 360 µL; working well volumes of 75 to 200 µL. -1 x 8 strips are designed to fit only one way into the strip holder, eliminating the chance of misorientation. -Lids are not available for these microplates.

(0)

Links

Corning Life SciencesCompany website

Tags

MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.

Related resources