Successful Application of Novel Plant ChIP-seq Technology Results in High Quality Sequencing Data from Limited Amounts of <i>Arabidopsis thaliana</i> Seedling Cells

The Bioruptor® Pico is the new next-generation all-in-one shearing device, highly optimized for DNA shearing for next-generation sequencing, chromatin shearing for ChIP-seq applications, RNA shearing, protein extraction, and cell/tissue lysis. The Pico is the shearing device of choice for sequencing applications, providing optimal yields, lengths, and consistency and excels in shearing different fragment size ranges, which are frequently required for downstream applications (e.g. bridge amplification) for sequencing. Moreover, the Pico provides flexibility in other applications saving the need to buy additional equipment. Unlike competing systems, the Pico has high throughput capabilities and can process up to 12 samples at once. The only ultrasonicator you’ll ever need, optimized for: Next-Generation Sequencing (5 - 100 µl) Chromatin shearing (10 µl - 2 ml) RNA shearing Protein extraction Tissue and cell lysis Simultaneous sonication of 12 samples Small and light  

The iDeal ChIP Kit for Transcription Factors.Diagenode’s iDeal ChIP-seq Kit for Transcriptions Factors is a highly specialized solution for robust ChIP-seq results transcription factors. Unlike competing solutions, our kit utilizes a highly optimized protocol and is backed by validation with a broad number and range of transcription factors. The kit provides high yields with excellent specificity and sensitivity. Advantages over competing solutions: Confidence in results: Validated for ChIP-seq with multiple transcription factors Proven: Validated by the epigenetics community, including the BLUEPRINT consortium Most complete kit available for highest quality data -- includes controls, primers, and magnetic beads Validated with Diagenode’s MicroPlex Library Preparation Kit and IP-Star Automation Completely automatable

The easiest NGS Library preparation you will ever find! The MicroPlex Library Preparation™ kit is the only kit on the market which is validated for ChIP-seq and which allows the preparation of indexed libraries from just picogram inputs. In combination with the True MicrochIP kit, it allows for performing ChIP-seq on as few as 10,000 cells. Studies showed a perfect match between the top 40% of peaks and the reference ChIP-seq dataset. Based on the NIH Encode project criterion, ChIP-seq results are considered reproducible between an original and reproduced dataset if the top 40% of peaks have at least an 80% overlap ratio with the compared dataset. With less input, fewer steps, fewer supplies, and faster time to results, the MicroPlex kit is the library preparation solution of choice. MicroPlex Library Preparation™ Kit Features: Simplified protocol: single tube, 3-step protocol without any intermediate purifications 3 times faster than TruSeq™ - only 2 hours for entire protocol DNA sample inputs from only 50 pg Specifically optimized for ChIP-seq Fewer PCR cycles: less bias Indexing options, easily automated Proven accuracy and reproducibility  Diagenode’s MicroPlex Library Preparation Kits v3 have been extensively validated for ChIP-seq samples and are optimized to generate DNA libraries with high molecular complexity from the lowest input amounts – down to 50 pg. The kit MicroPlex v3 includes all buffers and enzymes necessary for the library preparation. For flexibility of the choice different formats of compatible dual indexes are available separately.