ResourceSeparations
Speciation of iron(II) and iron(III) applying post-column reaction and subsequent UV/VIS detection
21 Nov 2012Speciation analysis is an important tool in analytical chemistry giving information about the quantitative distribution of different oxidation states of one and the same metal ion. In this application note the speciation of iron(II) and iron(III) (Fe2+/Fe3+) is achieved by ion chromatographic separation of their anionic dipicolinic acid complexes. The post-column reaction with 4-(2-)pyridylazo-resorcinol (PAR) allows VIS detection at 510 nm.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MetalsMetal analysis is critical in various industries, including environmental monitoring, food safety, and pharmaceuticals. Techniques such as ICP-MS and atomic absorption spectrometry are commonly used to detect trace metals. Explore metal analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.