Spatial profiling of immune cell gene signatures in the tumor microenvironment

Complex tissues with high cellular heterogeneity require single-cell technologies both at the transcriptomic and spatial level to fully interrogate the cell types within them. This application note demonstrates the capabilities of a multiplexed in situ transcriptomic approach for the spatial mapping of target genes in highly complex and heterogenous FFPE tumor tissues using the RNAscope HiPlex v2 assay.