Sorting of Fluorescent Protein Expressing Cells Using the Sony SH800S Cell Sorter
14 Oct 2018Fluorescent proteins (FP) are used as reporter molecules in a variety of studies analyzing protein expression and gene editing properties of CRISPR and other nucleases. Since the characterization and sequencing of the green fluorescent protein (GFP), a wide pallet of spectrally distinct FPs have been discovered. This broad range of FPs allow researchers to generate cells expressing a unique fluorescent protein or combinations
of them.
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SH800 Cell Sorter
Sony BiotechnologyThe benchtop SH800 cell sorter permits sorting of a wide range of cell sizes for many applications using the 70-µm, 100-µm, and 130-µm microfluidic sorting chips. This novel, chip-based design is fully integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis to make sorting less subjective, more precise and easier to perform. System software is intuitive and supports sorting into tubes and 96- and 384-well plates.