Simultaneous Determination of Metformin and its Chloride Counterion Using Multi-Mode Liquid Chromatography with Charged Aerosol Detection

This work demonstrates the determination of metformin (API) and its counterion (chloride) using a simple isocratic method. Thermo Scientific™ Acclaim™ Trinity™ P2 columns offer cation-exchange, anion-exchange and HILIC retention mechanisms on the same phase, thus they are ideal for separating both cationic and anionic species simultaneously.