Simplified Large Scale Production of Influenza A Virus Using the Corning® HYPERFlask® Cell Culture Vessel

20 Jan 2014

MDCK cells are commonly used in laboratories for the production of influenza A virus. This study validates use of the Corning HYPERFlask cell culture vessel for the large-scale production of influenza A virus. Results are compared to a conventional T-175 flask.

Corning® CellBIND® Surface HYPERFlask® Cell Culture Vessel

Corning Life Sciences

The High Yield PERformance Flask (HYPERFlask) cell culture vessel has 1720 cm2 available growth area on a polystyrene gas permeable surface. Treated for optimal cell attachment and gamma sterilized. Bar coded and compatible for use on Cell Culture Automation Platforms. Solid cap with liner on a straight neck designed for filling with growth medium. Made with USP Class VI Materials – molded and assembled in a Certified Class 100,000 clean room Sterile – gamma irradiated to sterility assurance level (SAL) 10⁻⁶ Clean Room Packaging – each pack is double bagged. Innovative 10Layer Design – 10 interconnected polystyrene growth surfaces Optimal Growth – Corning CellBIND® Surface treated gas permeable polystyrene for superior cell attachment and growth Increase Cell Yield – 10-fold higher cell yield increases productivity and capacity Time and Space Savings – Reduce processing time and incubator storage space by handling one flask compared to 10 traditional 175 cm2 flasks

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Simplified Large Scale Production of Influenza A Virus Using the Corning® HYPERFlask® Cell Culture Vessel