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Separation of Lipids by Normal Phase Flash Chromatography

Separation of Lipids by Normal Phase Flash Chromatography

9 Jul 2015

Lipid mixtures can be difficult to separate and frequently require multiple procedures to achieve a high purity. This application note demonstrates how this difficult procedure can be performed easily and efficiently with an Isco Normal Phase RediSep® column and CombiFlash® chromatography system. For this study, a mixture was prepared containing non-polar lipids, glycolipids, and phospholipids.

CombiFlash® Torrent

Teledyne Labs

Get the ease of use and reliability provided by CombiFlash and RediSep for Development Scale purification with the CombiFlash Torrent Purification System from ISCO. One liter per minute flow rates rapidly purify up to 300 grams of sample. The 100 psi pumps accurately deliver a reproducible binary gradient. The CombiFlash Torrent system is designed to be flexible for a variety of large-scale purification needs, with various methods of sample introduction and collection.

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Separation of Lipids by Normal Phase Flash Chromatography