Separation of Intact Monoclonal Antibody Sialylation Isoforms by pH Gradient Ion-Exchange Chromatography

Strong and Weak Cation-Exchange Columns for High-Resolution Separation of Proteins and MAbsThe Thermo Scientific ProPac™ SCX column line has expanded to include the next generation ProPac SCX-20 column. This column provides unequalled high resolution of protein variants.ProPac SCX-10 and WCX-10 strong and weak cation-exchange columns are based on a nonporous core particle that results in high-efficiency peaks and high-resolution separations of protein variants. A hydrophilic layer prevents unwanted secondary interactions. A grafted cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.ProPac® SCX and WCX Protein Column Features: Characterization and quality control assessment of monoclonal antibodies and other proteins Unequalled resolution High-resolution separations with excellent sample recovery Unmatched column-to-column and lot-to-lot reproducibility Useful for characterization of related protein variants (e.g., deamidation and MAb lysine truncation variants) Highly-efficient peaks with excellent sample recovery Available in different dimensions and formats for flexibility in method development Column Details: Length: 50, 100, 150, 250 mm ID: 2.0, 4.0, 9.0, 22 mm