Sensitive cfDNA sequencing using adaptive PCR control with icon96

This application note describes how adaptive, real‑time PCR amplification using the icon96™ thermocycler with AutoNorm™ technology enables highly sensitive sequencing of cell‑free DNA (cfDNA) from ultra‑low input samples. Developed in collaboration with the Dana‑Farber Cancer Institute, the workflow demonstrates uniform library yields across variable inputs, improved on‑target capture efficiency, and reliable detection of variants down to 0.5% allele frequency, even from just 1 ng of cfDNA.