ResourceSeparations

Rapid Separation of Anthocyanins in Cranberry and Bilberry Extracts Using a Core-Shell Particle Column

22 May 2016

This application note presents compares two HPLC methods for the qualitative analysis of bilberry extract, which is a complex sample with 15 anthocyanins and five anthocyanidins and the quantitative analysis of cranberry extract. The advantages of using a column that it is packed with solid core-porous shell particles, one of the newer developments in separation science, are described.

Thermo Scientific™ Acclaim™ 120 C18 Reversed-Phase LC Column

Thermo Fisher Scientific

High Performance Reversed-Phase Columns for the Separation of Small Molecules. The Acclaim® 120 columns are designed for high resolution reversed-phase separations. The very high surface coverage and very low metal content together result in columns with excellent efficiencies. These columns provide exceptional performance for a variety of applications in the pharmaceutical, chemical, environmental, and food separations areas.Acclaim 120 C18 columns have high surface coverage, resulting in high-capacity columns. They are stable between pH 2 and 8 and available in 3 and 5 μm particles sizes, and 4.6, 3.0, and 2.1 mm-diameters, with an average pore diameter of 120 Å. Acclaim 120 C18 is also available in 2.2µm RSLC format (see Acclaim Rapid Separation LC columns section for more details). All phases are LC/MS compatible because of the low silica bleed that results from the stable bonding procedure.Acclaim® 120 C18 Reversed-Phase LC Column Features: Ultrapure silica with high-density bonding for low silanol activity Very high efficiencies for maximum resolution Reproducible manufacturing practices for reproducible column-to-column performance LC/MS compatible Column Details: Particle Size: 3, 5 µm Pore Size: 120 Å Length: 50, 100, 150, 250 mm ID: 2.1, 3.0, 4.6 mm

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Rapid Separation of Anthocyanins in Cranberry and Bilberry Extracts Using a Core-Shell Particle Column