Quantitation of γ-H2AX Spots on the ImageStream
6 Feb 2011This application note shows ImageStream methods for quantifying DSB foci using automated γ-H2AX and 53BP1 spot counting, both in tumor cell lines and in primary murine CD8+ T cells. We also show quantification of MDC and γ-H2AX colocalization at DSB foci following irradiation.
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