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Protein Separation with pH Gradients Using Composite Buffer Systems Calculated by the Agilent Buffer Advisor Software

6 Aug 2020

This Technical Overview by Agilent provides some tips and tricks on how to correctly apply the Agilent Buffer Advisor Software for the generation of pH gradients over a wide range

Agilent 1260 Infinity II Binary Pump

Agilent Technologies

The 1260 Infinity II Bio-Inert Pump is a quaternary UHPLC pump that is made of bio-inert material for use in biological and extreme pH applications. This pump enables gradient formation and solvent blending from up to four solvents and operates at pressures up to 600 bar and flow rates up to 10 mL/min.

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Protein Separation with pH Gradients Using Composite Buffer Systems Calculated by the Agilent Buffer Advisor Software