SelectScience
Join Free
ResourceSeparations

Principles and solutions of size exclusion chromatography

Cytiva
29 Jul 2025

Size exclusion chromatography (SEC) is a highly versatile separation technique. It is well suited for biomolecules sensitive to changes in pH, concentration of metal ions or cofactors, or harsh environmental conditions. Separations can be performed in the presence of essential ions, cofactors, detergents, urea, or guanidine hydrochloride at high or low ionic strength. Furthermore, SEC can be performed within a wide temperature range covering the requirements of most experiments.

Explore the use of SEC for the purification and separation of biomolecules. Discover how you can optimize results focusing on SEC resins and find common applications and theoretical principles behind this technique.

Resource details:

Resource type: Application Notes
Page count: 108
Read time: 2 hours 42 mins

Links

CytivaCompany website

Tags

Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomoleculesChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.

Related resources

Principles and solutions of size exclusion chromatography - SelectScience resources