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Practical Tips for Cellular Quantification and Imaging Using Thermo Scientific Varioskan LUX Multimode Reader and Invitrogen EVOS FL Auto Cell Imaging System
8 Mar 2017Download this informative technical note to discover practical tips for facilitating the use of Thermo ScientificTM VarioskanTM LUX multimode reader and InvitrogenTM EVOSTM FL Auto Cell Imaging System, with the goal of achieving successful cellular quantification and imaging of mammalian cells in microplate-based assays.
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Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader
Thermo Fisher ScientificTop-of-the-line modular and upgradeable multimode reader with up to five detection technologies. Experience simplified versatility with powerful, intuitive Thermo Scientific SkanIt Software
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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.