Postcolumn Reactions Enhance Detection Sensitivity and Selectivity in HPLC Analyses
Postcolumn Reactions Enhance Detection Sensitivity and Selectivity in HPLC Analyses
5 Jul 2015HPLC analyses of barbiturates can be done rapidly with little sample preparation. Detection, however, presents a set of conflicts, leading to increased likelihood of interference from other sample components. The application note demonstrates a procedure to form a molecular species from an analyte for which the detector has higher sensitivity, or to enable the use of more selective conditions (e.g., a different UV wavelength or fluorescence).
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Ascentis® C18 HPLC column, 3micron, 15cm L x 4.6mm ID
Sigma-Aldrich SupelcoThe Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide. The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.