Optimizing and monitoring AAV capsid purification using mass photometry

This application note details Refeyn's collaboration with Pharmaron Gene Therapy to demonstrate the capabilities of the Samux^MP mass photometer in adeno-associated virus (AAV) analysis. The Samux^MP is optimized for AAV analysis and proves instrumental in process development and downstream purification monitoring. It quantifies the proportions of empty and full capsids and detects impurities. Using mass photometry technology, the Samux^MP accurately measures individual particle masses regardless of size or shape, providing quick results with minimal sample requirements. Pharmaron Gene Therapy utilized the Samux^MP to analyze AAV samples at various development stages, from harvest to chromatographic column separation.