Opera Phenix High Content Screening System Technical Performance: Improved 3D Imaging
22 May 2017Spinning disk confocal microscopy is a common tool for live cell microscopy and reduces background fluorescence from out of focus planes. However, for 3D imaging, confocal image quality can be limited by a phenomenon known as ‘pinhole crosstalk’. Out-of-focus light and scattered emission bleeds through neighboring pinholes and increases the background haze. This can lead to blurred images in conventional spinning disk systems. The confocal scanning unit of the Opera Phenix™ High Content Screening System provides an increased distance between pinholes to reduce the pinhole crosstalk. This leads to improved 3D image quality.
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Opera Phenix High Content Screening System
RevvityThe Opera Phenix ® Plus High-Content Screening System is the premier confocal solution for today' most demanding high content applications. Drawing on over a decade of experience with the industry-leading Opera ® and Opera ® Phenix system, the Opera Phenix Plus is designed for high-throughput high-content assays, phenotypic screening, assays using complex disease models, such as live cells, primary cells and microtissues, and fast-response assays, such as Ca2+ flux.