One-Step Triglyceride Separation by Reversed Phase HPLC Without Argentation
One-Step Triglyceride Separation by Reversed Phase HPLC Without Argentation
1 Jul 2015This application note demonstrates a rapid-one step process for the separation of saturated and unsaturated fatty acids in triglycerides. This method separates triglycerides by both acyl chain length and degree of unsaturation.
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Ascentis® C18 HPLC column, 3micron, 15cm L x 4.6mm ID
Sigma-Aldrich SupelcoThe Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide. The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.