One-shot protein engineering with Ribbon's MiroSynth DNA in a rapid and integrated cell-free workflow

This whitepaper presents a streamlined approach to protein engineering using a fully cell‑free workflow enabled by MiroSynth™ Cell‑Free DNA. By integrating computational design, high‑accuracy DNA synthesis, and multiplex protein expression screening, the method reduces the time and complexity of traditional design‑build‑test cycles. The workflow demonstrates how rapid, cell‑free DNA production and expression can improve protein stability, yield, and scalability, offering a faster route to optimized protein variants for biopharmaceutical and research applications.