Novel thermodynamic assay for biomolecular structure stability

This application note demonstrates a thermodynamic approach to assessing biomolecular structure stability using nano differential scanning calorimetry. The method shows how subtle changes in formulation conditions, such as pH and excipient concentration, can significantly influence protein stability, with high sensitivity and reproducibility. Using the Nano DSC system, the study highlights how thermodynamic profiling enables precise detection of structural changes without the need for sample labeling or modification, supporting more informed decisions in drug development, formulation optimization, and storage stability monitoring.