Non-targeted profiling of polar metabolites in human plasma

A particular challenge for chromatography is to find the balance between sensitivity and covering a broad spectrum of substances. Hydrophilic liquid chromatography (HILIC) is therefore especially suitable for such analyses containing polar compounds. It works with mass spectrometry (MS) and has very good chromatographic performance. With this combination, non-targeted analysis is well suited to the screening of polar metabolites in human plasma. YMC Europe highlights the non-targeted screening of polar metabolites in human plasma using a bioinert YMC-Accura Triart Diol-HILIC column. The bioinert coated stainless-steel hardware is essential to achieve the highest sensitivity and chromatographic resolution of endogenous isomers.