Multiplex Sequence Capture for Targeted Resequencing of Candidate Gene Panels in Cancer

Complex, progressive, multigenic, somatic mutation of the genome is now widely accepted as the primary driving force in the evolution of tumor initiation, growth, metastasis and pharmacoresistance for many types of cancer. Different types of tumors, at different stages of progression, are associated with mutations in distinct sets of genes, and there is a pressing need for a sequencing-based method of analyzing panels of candidate genes that can differ for each tumor type. This application note demonstrates the application of multiplex Sequence Capture techniques using custom gene panels for the analysis of somatic genome variation in colorectal tumors.