Multiplex data utilizing a single-step staining and imaging workflow to investigate multiple sample types

Understanding the tumor microenvironment has proven particularly important for oncology and immune oncology studies. Resolving tissue complexity across a statistically relevant number of patient biopsies at a cellular level has historically been challenged by image resolution, the number of targets that can be simultaneously assessed and sample throughput. These barriers have been broken with the generation of high plex, whole-slide immunofluorescence (IF) imaging data in a single cycle using the Orion™ platform to deliver multiplex biomarker quantitation in just hours. RareCyte gives some specific examples of how the Orion one-step stain-and-scan approach has been used to rapidly generate high-quality, subcellular quantitative IF data for multiple biomarkers across whole tissue sections. Plus, RareCyte describes how this, combined with traditional H&E on the same platform, can be used to provide accretive insights across the same cells and tissue microenvironments from the same biopsy sample.