ResourceSeparations
MS/MS<sup>ALL</sup> with SWATH<sup>TM</sup> Acquisition for Targeted Quantitation and Unknown Screening of Pesticides
11 Dec 2014This poster demonstrates a generic information-independent acquisition workflow to perform targeted quantitation and unknown screening of pesticides. Fruit and vegetable samples were extracted using QuEChERS approach, and then subjected to a generic RPLC separation. High resolution TOF MS data was acquired using a MS/MSALL with SWATHTM (Sequential Windowed Acquisition of all Theoretical Masses) method.
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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.PesticidesPesticides are used to protect crops from diseases, pests and unwanted plants. They are classed as persistent organic pollutants (POPs) and can be harmful to humans. The Environmental Protection Agency (EPA) currently regulates the use of pesticides in the USA. Food TestingFood testing refers to a variety experiments including PCR, mass spectrometry, processing, QuEChERS, analyzing and Kjeldahl.Data AcquisitionData acquisition refers to the collection and organization of information and data sets.