Microinjection into Plant Cells of Etiolated Seedlings Using the Eppendorf InjectMan® 4

22 Jun 2015

For several model systems microinjection is an established method to introduce DNA into single cells to generate transient and stable transformants. Not only nucleic acids but also proteins and small effectors can be injected also in combination to analyze e.g. cellular signal transduction events. This application note presents the greater efficacy of Eppendorf’s new micromanipulation system in increasing the survival rate of injected plant cells.

InjectMan® 4 Micromanipulator

Eppendorf

The new InjectMan® 4 is ideal for microinjection into adherent cells, smaller organisms and embryos in the early stages of development. The combination of InjectMan 4, FemtoJet® 4i or 4x enables a fast, semi-automatic injection. Furthermore, the InjectMan 4 is the ideal micromanipulator for all complex applications that require a dynamic movement mode and direct control of the injection process via the joystick button. The axial movement ensures the optimal protection of sensitive cells and lowest possible mortality rate. The high advancing speed makes penetration in rigid structures easier than ever. Maximum stability and highest precision ensures accurate and drift-free work. The new user interface with four pre-defined application masks facilitates and eases the individual workflow process (e.g. for adherent cell injection, serial microinjection into fish larvae, etc.). The freely programmable "My app" mask can be optimized for specific individual needs. Features: Maximum stability ensures drift-free work Selection and programming of additional functions (e.g. axial movement, step injection) Ergonomically shaped control panel for fatigue-free work Easy and precise angle adjustment Connection with Eppendorf PiezoXpert for piezo-supported penetration over a pre-defined distance Can be adapted to all conventional microscopes PC interface for remote control Applications: Semi-automatic microinjection into adherent cells Serial microinjection into fish larvae, frog eggs, insect embryos, etc. Injection into C. elegans and other roundworms and flatworms Automatic dispensing of cell suspensions or solutions

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