Methods for 16S rRNA sequencing using Illumina platforms

This guide provides an overview of 16S rRNA amplicon sequencing for taxonomic profiling of bacterial communities and microbiomes using Illumina next‑generation sequencing (NGS) platforms. It explains the principles of 16S rRNA gene sequencing, compares amplicon sequencing with shotgun metagenomics, and outlines a step‑by‑step workflow covering library preparation, sequencing, and data analysis for microbiome diversity studies.