High-throughput cell line development using CellCelector next-generation CLD nanowell plates

This case study demonstrates how Sartorius CellCelector next generation CLD nanowell plates enable high‑throughput screening, identification, and selection of monoclonal cell lines within a cell line development workflow. Using CHO K1 and HEK 293 suspension cell lines, the study shows that next-generation nanowell plates significantly increase the number of monoclonal wells detected, improve clone outgrowth, and reduce conflict wells compared with standard nanowell plates, offering a scalable and image‑verified alternative to limiting dilution and FACS‑based cloning methods.