Magnetic Bead Based Soil DNA Isolation Using the Thermo Scientific KingFisher Flex and KingFisher Duo and ClearMag™ Technology

19 Jan 2015

Certain sample materials offer more challenges in DNA purification than the others. Soil samples, for instance, contain lot of PCR inhibitors, which are difficult to remove during sample preparation. The PowerMag™ Soil DNA Isolation Kit was used together with the Thermo Scientific KingFisher Flex and KingFisher Duo magnetic particle processors for DNA purification from several different soil samples. The aim was to optimize an efficient sample preparation system ensuring high yield and quality DNA without inhibitors.

Thermo Scientific KingFisher Flex

Thermo Fisher Scientific

The Thermo Scientific KingFisher Flex magnetic particle processor offers great flexibility for high-throughput purification of DNA/RNA, proteins and cells from virtually any source.Using patented magnetic particle separation technology, the KingFisher Flex provides a fast and easy way to get high yields of purified samples with excellent reproducibility and quality.Key Features: High-throughput system processes up to 96 samples in 30 minutes Flexible, open system lets you customize your own purification process Patented magnetic particle processing technology lets you set your protocols and walk away Application Areas: Genetic testing Microbial identification Plant genetics Evolutionary studies / Phylogenetics Protein identification Immunology

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Thermo Scientific KingFisher Duo

Thermo Fisher Scientific

The Thermo Scientific KingFisher Duo magnetic particle processor and KingFisher optimized reagent kits provide everything you need to get high yields of quality nucleic acid for downstream applications. The compact KingFisher Duo instrument is ideal for space-restricted laboratories that are looking to reduce hands-on time required for purifying DNA/RNA, cells or proteins. It can be used with a variety of starting materials, from blood and other body fluids to cells and tissue samples, making the system flexible for a variety of applications.Key Features: Compact instrument with a small bench top footprint Improves reproducibility and saves time over manual purification methods Processes a variety of sample types in volumes ranging up to 5mL Optimized DNA and RNA isolation kits and protocols for pure performance, every time Application Areas: Genetic testing Microbial identification Plant genetics Evolutionary studies / Phylogenetics Protein identification Immunology

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