Light-sheet fluorescence microscopy for living plants

17 Jul 2023

Imaging living plants involves challenges on several levels, including sample properties (e.g., aerial and roots, chlorophyll, cell walls), tissue visualization (e.g., dyes vs reporter lines), sample-friendly imaging (e.g., embedding, low phototoxicity, timelapses), and efficient data handling (e.g., processing and storage). Working together with end users and application specialists, Bruker Luxendo has the experience needed to deliver expert commercial LSFM solutions that can significantly aid the imaging of living and naturally growing plants. In this application note, Bruker highlights challenges with using microscopy for image acquisition of plants, challenges specific to LSFM, data processing challenges, and how Bruker Luxendo can support you in overcoming these obstacles to create meaningful data.

MuVI SPIM

Bruker Fluorescence Microscopy

Bruker's MuVi Selective-Plane Illumination Microscope (SPIM) is the fastest multi-angle view system on the market. MuVi incorporates years of Luxendo light-sheet technology innovations to provide high-speed volumetric acquisition for analyzing dynamic processes in delicate live specimens, as well as offering compatibility with any clearing method. A variety of other advanced hardware and software integrations can be easily configured to support evolving multidimensional experimental needs.

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