Light-sheet fluorescence microscopy for living plants

17 Jul 2023

Imaging living plants involves challenges on several levels, including sample properties (e.g., aerial and roots, chlorophyll, cell walls), tissue visualization (e.g., dyes vs reporter lines), sample-friendly imaging (e.g., embedding, low phototoxicity, timelapses), and efficient data handling (e.g., processing and storage). Working together with end users and application specialists, Bruker Luxendo has the experience needed to deliver expert commercial LSFM solutions that can significantly aid the imaging of living and naturally growing plants. In this application note, Bruker highlights challenges with using microscopy for image acquisition of plants, challenges specific to LSFM, data processing challenges, and how Bruker Luxendo can support you in overcoming these obstacles to create meaningful data.

MuVI SPIM

Bruker Fluorescence Microscopy

Bruker's MuVi Selective-Plane Illumination Microscope (SPIM) is the fastest multi-angle view system on the market. MuVi incorporates years of Luxendo light-sheet technology innovations to provide high-speed volumetric acquisition for analyzing dynamic processes in delicate live specimens, as well as offering compatibility with any clearing method. A variety of other advanced hardware and software integrations can be easily configured to support evolving multidimensional experimental needs.

(0)

Links

Tags

Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence MicroscopyFluorescence microscopy has become an essential tool in biology, as well as in materials science. The application of many fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity. Using multiple fluorescence labels, different probes can simultaneously identify several target molecules.Plant SciencePlant science studies the biology of plants, including genetics, physiology, ecology, and biotechnology. Research in this field is essential for improving crop yields, disease resistance, and sustainability. Explore plant science tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.
Light-sheet fluorescence microscopy for living plants