iCell® Endothelial Cells –Assaying Barrier Function Using xCELLigence RTCA Cardio System Application Protocol
20 Jun 2013Proper regulation of endothelium barrier integrity is a fundamental feature of angiogenesis and vascular homeostasis. Dysregulation of this barrier has been associated with pathological conditions, such as inflammation and vascular diseases, tumor metastasis, and early stages in the pathogenesis of atherosclerosis. iCell® Endothelial Cells, derived from human induced pluripotent stem cells, exhibit
morphological, biochemical, and pathophysiological characteristics of a native human endothelium. The xCELLigence RTCA Cardio System (RTCA System) is a non-invasive, label free platform that utilizes impedance changes across the cell monolayer to indirectly measure cell-cell interaction, transient contractions, and cell layer permeability. This application Note describes how to handle iCell Endothelial Cells for use on the RTCA System and provides basic instructions for compound treatment, data acquisition, and analysis. Together iCell Endothelial Cells and the RTCA System enable in vitro screening of compound effects on human endothelium permeability.
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iCell Endothelial Cells
Cellular Dynamics InternationaliCell® Endothelial Cells, interior surface blood vessel cells derived from human induced pluripotent stem (iPS) cells facilitate vascular-targeted drug discovery, tissue regeneration, and other life science research. iCell® Endothelial Cells exhibit characteristic endothelial gene and protein expression (e.g., CD31, CD105, CD144, ZO-1, and von Willebrand Factor) and endothelial cell functions (e.g., tubular formation, acetylated LDL uptake, barrier function, and wound healing). These cells are an ideal in vitro model system that provides a reliable source of endothelial cells suitable for use in vascular biology research including angiogenesis, atherosclerosis, inflammation, and many other research areas. iCell® Endothelial Cells are shipped as cryopreserved suspensions of dissociated cells with medium supplement, specially formulated for optimal cell performance. iCell Endothelial Cells remain viable in culture for up to 5 passages, thus enabling assessment of both acute and longer-term testing.iCell® Endothelial Cells Benefits: Human Cells - Cells are differentiated from human pluripotent stem cells that provide an easily accessible and physiologically relevant model system for vascular-targeted drug discovery, tissue regeneration and other vascular biology research. Highly Pure Cell Population - Provides endothelial cell-specific responses to reference molecules. Homogenous and Reproducible Fully Functional Model - Cells exhibit standard biochemical characteristics of normal human endothelial cells, and demonstrate utility in a variety of assays including cell migration, cytokine stimulation, tubular formation, and impedance/barrier function. Easy to Implement - Cells are shipped as cryopreserved suspensions of dissociated cells. Simply thaw and use. Acute and Longer-term Testing - Cells remain viable in culture for up to 5 passages, thus enabling assessment of both acute and longer-term testing. iPS Cell-derived iCell® Endothelial Cells Applications: Cells are amenable to a wide array of assays including: Cell adhesion, Cell invasion and migration, Cell permeability, Cell proliferation, Cell viability, Impedance/transendothelial electrical resistance, Tubular formation.