High-throughput sample prep for extensive plasma proteomics

Plasma proteomics is vital for identifying health and disease biomarkers, but the complexity of plasma samples poses challenges. Tecan describes a scalable and rapid automation platform for preparing plasma samples for proteomic analysis using the ENRICH-iST HT reagent kit, the Fluent^® liquid handler, and the Resolvex^® A200. The automated ENRICH-iST workflow addresses challenges by enriching low-abundance proteins, facilitating deeper protein identification and quantification compared to manual methods. Tecan highlights the efficiency and robustness of the automated process, showing high-throughput capabilities with minimal user intervention. It demonstrates consistent and reproducible results with over 97% overlap in protein identifications between manual and automated methods, making it a powerful tool for extensive plasma proteome studies.