ENRICH-iST Plasma/Serum Preparation Kit
Fast, reliable, and reproducible enrichment of low-abundant proteins from plasma and serum, based on novel ENRICH technology and in-StageTip (iST) technology.
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Accessing low-abundant proteins of interest in plasma and serum samples is challenging. The high dynamic range in protein concentrations and the heterogeneity and complexity pose significant obstacles for LC-MS-based proteomics, limiting access to full proteome information. In consequence, results lack robustness and reproducibility, traditional workflows are complex and time-consuming and low-abundant proteins are masked by high-abundant proteins. Thus, the impracticality of automation, the lack of flexibility and the inability to produce unbiased results impede the application for large sample cohorts.
To over come these challenges ENRICH-iST kit offers:
- Streamlined enrichment of low abundance plasma proteins coupled with iST proteomic sample preparation technology for greater proteomic depth.
- Fast, easy-to-use and standardized protocol for high reproducibility and less hands-on time.
- Automatable high-throughput processing of up to 96 samples within 5 hours.
- Species-independent and flexible technology compatible with serum samples.
- Optimized for low input samples (20 µL) for large-scale proteomic studies.
High-throughput sample prep for extensive plasma proteomics
Plasma proteomics is vital for identifying health and disease biomarkers, but the complexity of plasma samples poses challenges. Tecan describes a scalable and rapid automation platform for preparing plasma samples for proteomic analysis using the ENRICH-iST HT reagent kit, the Fluent® liquid handler, and the Resolvex® A200. The automated ENRICH-iST workflow addresses challenges by enriching low-abundance proteins, facilitating deeper protein identification and quantification compared to manual methods. Tecan highlights the efficiency and robustness of the automated process, showing high-throughput capabilities with minimal user intervention. It demonstrates consistent and reproducible results with over 97% overlap in protein identifications between manual and automated methods, making it a powerful tool for extensive plasma proteome studies.
